期刊
VISION RESEARCH
卷 44, 期 4, 页码 339-348出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.visres.2003.09.032
关键词
gene therapy; retinal degeneration; photoreceptor; retinal pigment epithelium
We recently reported that different purification methods of recombinant adeno-associated virus type 2 (rAAV2) affect the transduction characteristics following subretinal injection. In this study, we examined the roles of contaminant proteins from the HEK-293 cells and helper adenovirus, inactivation of helper adenovirus and cell stress induced by DNA-damaging agents in rAAV-mediated retinal transduction. Our results showed that contaminating factors/proteins resulting from the helper E1 deleted adenovirus are possibly responsible for efficient RPE transduction. Future studies of these factors will undoubtedly lead to development of new therapeutic approaches to PR- and RPE-specific retinal diseases. (C) 2003 Elsevier Ltd. All rights reserved.
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