期刊
STRUCTURE
卷 12, 期 2, 页码 193-204出版社
CELL PRESS
DOI: 10.1016/j.str.2004.01.003
关键词
-
资金
- NHLBI NIH HHS [HL 59725] Funding Source: Medline
- NIAID NIH HHS [AI-36085] Funding Source: Medline
- NIGMS NIH HHS [GM-46192] Funding Source: Medline
447-52D is a human monoclonal antibody isolated from a heterohybridoma derived from an HIV-1-infected individual. This antibody recognizes the hypervariable gp120 V3 loop, and neutralizes both X4 and R5 primary isolates, making it one of the most effective anti-V3 antibodies characterized to date. The crystal structure of the 447-52D Fab in complex with a 16-mer V3 peptide at 2.5 Angstrom resolution reveals that the peptide beta hairpin forms a three-stranded mixed beta sheet with complementarity determining region (CDR) H3, with most of the V3 side chains exposed to solvent. Sequence specificity is conferred through interaction of the type-II turn (residues GPGR) at the apex of the V3 hairpin with the base of CDR H3. This novel mode of peptide-antibody recognition enables the antibody to bind to many different V3 sequences where only the GPxR core epitope is absolutely required.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据