期刊
BRITISH JOURNAL OF HAEMATOLOGY
卷 124, 期 4, 页码 481-484出版社
WILEY
DOI: 10.1111/j.1365-2141.2004.04808.x
关键词
FLT3; acute myeloid leukaemia; tyrosine kinase receptor; mutation
类别
资金
- NCI NIH HHS [K23 CA92405-01, CA18029] Funding Source: Medline
Internal tandem duplications in FLT3 are the most common mutation in acute myeloid leukaemia (AML), with agarose gel electrophoresis of polymerase chain reaction products (PCR/agarose) being the screening method of choice for these mutations. As PCR/agarose screening does not detect small mutations, single-stranded conformational polymorphism analyses (PCR/SSCP) were used in an attempt to identify previously unrecognized point mutations in FLT3 exons 14 and 15 of 140 AML patients, using newly designed primers that anneal within intron sequences. Novel missense point mutations were found in exon 14, suggesting additional investigations should be performed in AML and other haematopoietic malignancies, using this sensitive technique.
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