期刊
EUROPEAN JOURNAL OF BIOCHEMISTRY
卷 271, 期 4, 页码 694-702出版社
WILEY
DOI: 10.1111/j.1432-1033.2003.03971.x
关键词
methanogenic archaea; seryl-tRNA synthetase; tRNA dimerization; tRNA(Ser) recognition
The methanogenic archaea Methanococcus jannaschii and M. maripaludis contain an atypical seryl-tRNA synthetase (SerRS), which recognizes eukaryotic and bacterial tRNAs(Ser), in addition to the homologous tRNA(Ser) and tRNA(Sec) species. The relative flexibility in tRNA recognition displayed by methanogenic SerRSs, shown by aminoacylation and gel mobility shift assays, indicates the conservation of some serine determinants in all three domains. The complex of M. maripaludis SerRS with the homologues tRNA(Ser) was isolated by gel filtration chromatography. Complex formation strongly depends on the conformation of tRNA. Therefore, the renaturation conditions for in vitro transcribed tRNA(GCU)(Ser) isoacceptor were studied carefully. This tRNA, unlike many other tRNAs, is prone to dimerization, possibly due to several stretches of complementary oligonucleotides within its sequence. Dimerization is facilitated by increased tRNA concentration and can be diminished by fast renaturation in the presence of 5 mM magnesium chloride.
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