期刊
JOURNAL OF NEUROSCIENCE
卷 24, 期 5, 页码 1226-1235出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4286-03.2004
关键词
calcium imaging; calcium spark; intracellular calcium; neurosecretion; presynaptic; ryanodine receptor
资金
- NHLBI NIH HHS [HL61297, R01 HL061297] Funding Source: Medline
- NIDA NIH HHS [DA10487, R01 DA010487] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
Localized, brief Ca2+ transients (Ca2+ syntillas) caused by release from intracellular stores were found in isolated nerve terminals from magnocellular hypothalamic neurons and examined quantitatively using a signal mass approach to Ca2+ imaging. Ca2+ syntillas (scintilla, L., spark, from a synaptic structure, a nerve terminal) are caused by release of similar to250,000 Ca ions on average by a Ca2+ flux lasting on the order of tens of milliseconds and occur spontaneously at a membrane potential of -80 mV. Syntillas are unaffected by removal of extracellular Ca2+, are mediated by ryanodine receptors (RyRs) and are increased in frequency, in the absence of extracellular Ca2+, by physiological levels of depolarization. This represents the first direct demonstration of mobilization of Ca2+ from intracellular stores in neurons by depolarization without Ca2+ influx. The regulation of syntillas by depolarization provides a new link between neuronal activity and cytosolic [Ca2+] in nerve terminals.
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