期刊
CHEMBIOCHEM
卷 5, 期 2, 页码 231-239出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.200300734
关键词
alpha-amylase; glycoside hydrolase family-13; metal binding; salt bridge; thermostability
There are currently 35 available nonredundant molecular structures of class-13 alpha-amylases (EC3.2.1.1), mostly from microbial organisms living under a wide range of environmental conditions. One of the most recent additions has been the first a-amylose structure of a hyperthermophilic archaeon [Linden etal., J. Biol, Chem. 2003, 278, 9875-9884]. The structure has been used for comparative analyses with a representative set of three alpha-amylases from thermophilic, mesophilic and psychrophilic sources to identify molecular parameters for environmental adaptation. Our analysis supports generally observed trends such as an increase in structural compactness as well as on increase in salt bridges in order to cope with high-temperature conditions. The two representative thermophilic structures used in this comparative study have independently evolved di-metal centres-not present in the mesophilic and psychrophilic structures-in the vicinity of the active site. These observations may provide impetus for the design of alpha-amylases with improved molecular properties to enhance their utility in biotechnological processes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据