期刊
FEMS MICROBIOLOGY LETTERS
卷 231, 期 1, 页码 85-90出版社
OXFORD UNIV PRESS
DOI: 10.1016/S0378-1097(03)00934-0
关键词
nisin; lactococcus; nisI; nisin immunity; nisin enhancer
类别
Lactococcus lactis cells producing the antibacterial peptide nisin protect their own cytoplasmic membrane by specific immunity proteins, NisI and NisF/E/G. We show here that approximately half of the produced Nisi escaped the lipid modification (LF-NisI = lipid-free NisI) and was secreted to the medium, and that LF-NisI had no affinity to cells of L. lactis. The molar ratio of NisI and nisin was determined to be approximately 1:10 on the cell surface and 1:50 in the culture supernatant. Purified LF-NisI was shown to enhance the activity of nisin against several tested indicator strains. The enhancement of nisin activity by LF-NisI was not observed with cells containing the NisFEG transport system. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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