期刊
FEBS LETTERS
卷 559, 期 1-3, 页码 51-56出版社
WILEY
DOI: 10.1016/S0014-5793(04)00022-5
关键词
chlorella cell wall; chlorovirus; polysaccharide lyase; matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; vAL-1
Cell wall materials isolated from Chlorella cells were degraded by the polysaccharide-degrading enzyme vAL-1 encoded by chlorovirus CVK2. Matrix-assisted laser desorption/ ionization time-of-flight mass spectrometric analyses of the degradation products (oligosaccharides) revealed major oligosaccharides contain unsaturated GlcA at the reducing terminus, and a side chain attached at C2 or C3 of GlcA(C4 = C5), which mainly consisted of Ara, GlcNAc and Gal. The results indicated that vAL-1 is a novel polysaccharide lyase, cleaving chains of beta- or alpha-1,4-linked GlcAs. The unique structures of Chlorella cell wall were also revealed. Studies on the complicated structures of naturally occurring polysaccharides will be greatly facilitated by using vAL-1 as a tool in structural analysis. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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