期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 422, 期 2, 页码 161-167出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2003.11.023
关键词
chondrocyte; mechanotransduction; attachment; cytoskeleton; alginate; integrin
资金
- NIAMS NIH HHS [AR-07572] Funding Source: Medline
This study characterized the attachment of chondrocytes to RGD-functionalized alginate by examining the effect of substrate stiffness on cell attachment and morphology. Bovine chondrocytes were added to wells coated with 2% alginate or RGD-alginate. The alginate was crosslinked with divalent cations ranging from 1.25 to 62.5 mmol/g alginate. Attachment to RGD-alginate was 1020 times higher than attachment to unmodified alginate and was significantly inhibited by antibodies to integrin subunits alpha(3) and beta(1), cytochalasin-D, and soluble RGD peptide. The equilibrium level and rate of attachment increased with crosslink density and substrate stiffness. Substrate stiffness also regulated chondrocyte morphology, which changed from a rounded shape with nebulous actin on weaker substrates to a predominantly flat morphology with actin stress fibers on stiffer substrates. The dependence of attachment on integrins and substrate stiffness suggests that chondrocyte integrins may play a role in sensing the mechanical properties of the matrices to which they are attached. (C) 2003 Elsevier Inc. All rights reserved.
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