期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 63, 期 25, 页码 5976-5984出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b01505
关键词
galacto-oligosaccharides; sialic acid; trans-sialidase; Trypanosoma cruzi; bovine glycomacropeptide; bovine blood plasma glycoprotein
资金
- European Union
- European Regional Development Fund
- Ministry of Economic Affairs, Agriculture and Innovation, Peaks in the Delta
- Municipality of Groningen
- Provinces of Groningen, Fryslan, and Drenthe
- Dutch Carbohydrate Competence Center (CCC) [WP6]
Decoration of prebiotic galacto-oligosaccharides (GOS) with sialic acid yields mixtures of GOS and sialylated GOS (Sia-GOS), novel products that are expected to have both prebiotic and antiadhesive functionalities. The recombinantly produced trans-sialidase enzyme from Trypanosoma cruzi (TcTS), an enzyme with the ability to transfer (a2-3)-linked sialic acid from sialogalactoglycans to asialogalactoglycans, was employed to catalyze this sialylation. As sialic acid acceptor substrates, Vivinal GOS and derived fractions of specific degree of polymerization were taken. As sialic acid donor substrates, bovine ?-casein-derived glycomacropeptide [>99% N-acetylneuraminic acid (Neu5Ac); <1% N-glycolylneuraminic acid (Neu5Gc)] and bovine blood plasma glycoprotein mixture (45% Neu5Ac; 55% Neu5Gc) were selected, yielding potential food and feed products, respectively. High-pH anion-exchange chromatography, matrix-assisted laser-desorption ionization time-of-flight mass spectrometry, and nuclear magnetic resonance spectroscopy were used for product analysis.
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