期刊
VACCINE
卷 22, 期 7, 页码 818-821出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2003.11.026
关键词
Klebsiella; O antigen; immunotherapy
Prevention of Klebsiella infections by passive immunotherapy has received more attention during the last decade. Both K antigen-and o antigen-specific antisera and monoclonal antibodies (mAbs) have been studied with respect to phagocytosis-enhancing and in vivo protective capacities. Our own work has focussed on the generation of O serogroup-specific rabbit antiscra and O antigen specific murine antibodies, O-specific rabbit sera were absorbed extensively with heterologous O antigen strains in order to obtain highly specific typing reagents. Using these for typing a collection of 378 clinical strains, we found that 82% of them belonged to one of the four serogroups O1, O2ab, O3 and O5. Phagocytosis experiments using antisera and mAbs showed that O antigen specific antibodies were able to opsonize non-encapsulated strains, while fully encapsulated bacteria were rather resistant against the opsonizing effect. Nevertheless, in vivo experiments demonstrated a prophylactic effect on both Klebsiella septicemia and pneumonia in a mouse model of lethal infection. Given the limited numb er of O serogroups, O antigen-specific antibodies may be suited to supplement K antigen-specific hyperimmune globulins for passive immunoprophylaxis of Klebsiella infections. (C) 2003 Elsevier Ltd. All rights reserved.
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