期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 63, 期 23, 页码 5622-5633出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b00730
关键词
cranberry; arabino-xyloglucan; Escherichia coli; anti-adhesive; Urinary tract infection
资金
- Cooperative Research and Development Agreement
- Ocean Spray Cranberries, Inc. [58-3K95-1-1503]
Cranberry juice has been recognized as a treatment for urinary tract infections on the basis of scientific reports of proanthocyanidin anti-adhesion activity against Escherichia coli as well as from folklore. Xyloglucan oligosaccharides were detected in cranberry juice and the residue remaining following commercial juice extraction that included pectinase maceration of the pulp. A novel xyloglucan Was detected through tandem mass spectrometry analysis of an ion at m/z 1055 that was determined to be a branched, three hexose, four pentose oligosaccharide consistent with an arabino-xyloglucan structure. Two-dimensional nuclear magnetic resonance spectroscopy analysis provided through-bond correlations for the alpha-L-Araf (1 -> 2) alpha-D-Xylp (1 -> 6) beta-D-Glcp sequence, proving the S-type cranberry xyloglucan structure. Cranberry xyloglucan-rich fractions inhibited the adhesion of E coli CFT073 and UT189 strains to T24 human bladder epithelial cells and that of E. coli O157:H7 to HT29 human colonic epithelial cells. SSGG xyloglucan oligosaccharides represent a new cranberry bioactive component with E coli anti-adhesion activity and high affinity for type, 1 fimbriae.
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