Oxidative damage to DNA, p53 gene expression and p53 protein level in the process of aging in rat brain

Levels of 8-oxo2'dG (HPLC), p53 mRNA (PCR) and p53 protein (Western Blot) were estimated in four structures of rat brain, including grey matter (GM) of cerebral cortex, cerebral white matter (WM), cerebellum (C) and medulla oblongata (MO) of control (3.0-3.5-month-old) rats, 12- and 24-month-old rats. The level of oxidative DNA was statistically significantly higher in C of 24-month-old animals. Expression of p53 gene increased in C and also in the all other investigated brain parts, while the protein level of p53 was enhanced only in GM of 24-month-old rats. These data indicated that DNA oxidative damage and p53 gene expression increased significantly in aged brain. The higher expression of p53 gene in aged brain may suggest the activation of DNA repair processes. (C) 2003 Elsevier B.V. All rights reserved.