Antineoplastic agents.: 520.: Isolation and structure of irciniastatins A and B from the Indo-Pacific marine sponge Ircinia ramosa

The Indo-Pacific marine sponge Ircinia ramosa has been found to contain two powerful (GI(50) from 0.001 to <0.0001 mug/mL) murine and human cancer cell growth inhibitors. Both were isolated (10(-3)-10(-4)% yields) by cancer cell line bioassay-guided techniques and named irciniastatins A (1) and B (2). Structural elucidation by a combination of spectral analyses, primarily high resolution mass and 2D-NMR (principally APT, HMQC, HMBC, and ROESY) spectroscopy, revealed the unusual structures 1 and 2.