期刊
FEBS LETTERS
卷 560, 期 1-3, 页码 69-74出版社
WILEY
DOI: 10.1016/S0014-5793(04)00073-0
关键词
modified DNA enzyme; N3'-P5' phosphoramidate oligonucleotide; influenza A virus; kinetic analysis; nuclease resistance; plaque formation assay
DNA enzymes are RNA-cleaving single-stranded DNA molecules. We designed DNA enzymes targeting the PB2 mRNA translation initiation (AUG) region of the influenza A virus (A/PR/8134). The modified DNA enzymes have one or two N3'-P5' phosphoramidate bonds at both the 3'- and 5'-termini of the oligonucleotides, which significantly enhanced their nuclease resistance. These modified DNA enzymes had the same cleavage activity as the unmodified DNA enzymes, determined by kinetic analyses, and reduced influenza A virus replication by more than 99%, determined by plaque formation. Theme DNA enzymes are highly specific; their protective effect was not observed in influenza B virus (B/Ibaraki)-infected Madin-Darby canine kidney cells. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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