期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 63, 期 31, 页码 7032-7040出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b02523
关键词
tyrosinase; 4-hexylresorcinol; antibrowning lightening hydrogen peroxide; kinetic characterization
资金
- MINECO, Madrid [SAF2013-48375-C2-1-R]
- University of Murcia, Murcia [UMU15452, UMU17766]
- MEC-FPU fellowship [AP2010-4300]
4-Hexylresorcinol (HR) is a compound used in the food and cosmetic industries as an antibrowning and lightening agent. Its use is mainly attributed to its inhibitory effect on the enzyme tyrosinase. However, the enzyme hydroxylates HR to an o-diphenol, which it then oxidizes to an o-quinone, which rapidly isomerizes to p-quinone. For tyrosinase to act in this way, the E-ox form (oxy-tyrosinase) must be present in the reaction medium, which can be brought about by (a) hydrogen peroxide, (b) ascorbic acid, or (c) catalytic concentrations of o-diphenol and a reductant (NADH) to maintain it constant. This work demonstrates that HR is a substrate of tyrosinase and proposes a mechanism for its action. Its kinetic characterization provides a catalytic constant of 0.85 +/- 0.04 s(-1) and a Michaelis constant of 60.31 +/- 6.73 mu M.
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