期刊
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
卷 40, 期 2, 页码 129-134出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1023/B:ABIM.0000018914.08571.50
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Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside (in order to remove bitterness and aftertaste), with cyclodextrins (CDs) being used as donors. It was shown that CGTases produced by extremophilic microorganisms are effective biocatalysts. Optimum temperature and pH of these enzymes were 45degreesC and pH 6.5-7.5, respectively. The optimum stevioside-to-CD ratio and total concentration of dry matter for the synthesis of the best-tasting product were 1 : 1 (w/w) and 11.6%, respectively.
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