期刊
JOURNAL OF BIOSCIENCES
卷 29, 期 1, 页码 67-71出版社
INDIAN ACAD SCIENCES
DOI: 10.1007/BF02702563
关键词
cotton (Gossypium); gossypol; high-performance liquid chromatography (HPLC); pigment gland
类别
A comparative study on gossypol content of various genetic types of pigment glands of cotton varieties was conducted through an optimized high-performance liquid chromatography (HPLC) on a C-18 column (4(.)6 mm x 250 mm, 5 mum particle) with methanol-0(.)5% acetic acid aqueous solution, 90 : 10 (v/v), as mobile phase, at a flow rate of 0(.)8 ml/min and UV detection at 254 nm. The method was shown to be highly reproducible, with precision [as relative standard deviation (RSD)] and accuracy [as relative mean error (RME)] <10%, both intra-day and inter-day. Absolute recoveries were >94%. The results revealed major differences among the different gland varieties or species of cotton, including the special and ordinary glandless and glanded Gossypium hirsutum, G. barbadense, and displayed the precious resources of different glands of extraordinary cotton.
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