期刊
EXPERIMENTAL PARASITOLOGY
卷 106, 期 3-4, 页码 183-186出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2004.03.009
关键词
Echinococcus granulosus; cestode; host-protective antigen; vaccine; gene family; gene expression; DNA; deoxyribonucleic; acid; cDNA; complementary DNA; PCR; polymerase chain reaction; UTR; untranslated region; RNA; ribonucleic acid; mRNA; messenger RNA; RT-PCR; reverse transcription-PCR
类别
A recombinant antigen vaccine has been developed which is effective in preventing the hydatid parasite, Echinococcus granulosus from infecting its animal intermediate hosts. The vaccine antigen, designated EG95, is expressed by a cDNA cloned from E. granulosus oncosphere mRNA. The gene encoding EG95 belongs to a small gene family where six of the members are transcribed in the oncosphere. Conditions were established which allowed specific RT-PCR amplification of mRNA for each gene family member and these conditions were applied to determine transcription patterns for each gene family member in gravid adult worms, oncospheres, and protoscoleces. The four eg95 gene family members which encode an identical EG95 protein, were transcribed only in the oncosphere. In contrast, two gene family members that encode variant EG95 proteins did not have transcription patterns confined to the oncosphere. These findings suggest a common biological role for four genes in the gene family and a separate role for the other, more variant gene family members. (C) 2004 Elsevier Inc. All rights reserved.
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