4.7 Article

Production of Long-Chain α,ω-Dicarboxylic Acids by Engineered Escherichia coli from Renewable Fatty Acids and Plant Oils

期刊

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 63, 期 37, 页码 8199-8208

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b03833

关键词

alpha,omega-dicarboxylic acids; CYP450s; monooxygenase; omega-oxidation pathway; fatty acids

资金

  1. National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning (MSIP) [NRF-2009-C1AAA001-2009-0093491]
  2. Next-Generation Bio Green 21 Program (SSAC) [PJ011181]
  3. Rural Development Administration, Republic of Korea
  4. National Research Foundation of Korea [22A20130012653, 2009-0093491] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

向作者/读者索取更多资源

Long-chain alpha,omega-dicarboxylic acids (LDCAs, >= C12) are widely used as a raw material for preparing various commodities and polymers. In this study, a CYP450-monooxygenase-mediated omega-oxidation pathway system with high omega-regioselectivity was heterologously expressed in Escherichia coli to produce DCAs from fatty acids. The resulting engineered E. coli produced a maximum of 41 mg/L of C12 DCA and 163 mg/L of C14 DCA from fatty acids (1 g/L), following 20 h of whole cell biotransformation. Addition of a heme precursor and the hydroxyl radical scavenger, thiourea, increased product concentration (159 mg/L of C12 DCA and 410 mg/L of C14 DCA) in a shorter culture duration than that of the corresponding controls. DCAs of various chain lengths were synthesized from coconut oil hydrolysate using the engineered E. coli. This novel synthetic biocatalytic system could be applied to produce high value DCAs in a cost-effective manner from renewable plant oils.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据