期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
卷 137, 期 3, 页码 207-225出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cca.2003.12.004
关键词
acetazolamide; alkalization; carbonic anhydrase; histochemistry; methazolamide; midgut; mosquito larvae; O-18 isotope
资金
- NIAID NIH HHS [R01 AI45098] Funding Source: Medline
- NIGMS NIH HHS [GM 25154] Funding Source: Medline
We have previously demonstrated the involvement of carbonic anhydrase (CA) in the alkalization mechanism of the Aedes aegypti larval midgut. In this study, we used Hansson's histochemistry to examine the distribution of the enzyme in the midgut of six different species of mosquito larvae (Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, Culex nigripalpus, Ochlerotatus taeniorhynchus, Anopheles quadrimaculatus). Additionally, we quantitated CA content in the gastric caeca, anterior and posterior midgut of fourth instar larvae from these species using the O-18 isotope exchange method coupled to mass spectrometry. We also tested the effect of CA inhibitors such as methazolamide and acetazolamide in the alkalization of the midgut for these species. Our results indicate that CA is present in the larval midget of the species studied and that it appears to be associated with the posterior midgut and gastric caeca in some species and with the anterior midgut in others. CA inhibitors appear to have a profound effect on the alkalization mechanism of the midgut with lethal consequences for most of the species tested. (C) 2004 Elsevier Inc. All rights reserved.
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