4.2 Article

Rheology and microstructure of heat-induced egg yolk gels

期刊

RHEOLOGICA ACTA
卷 43, 期 2, 页码 184-195

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SPRINGER
DOI: 10.1007/s00397-003-0338-3

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gel point; egg yolk gelation; small amplitude oscillatory shear; differential scanning calorimetry; electron microscopy

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The evolution of native egg yolk undergoing a thermal-induced sol-gel transition was studied by using temperature controlled small amplitude oscillatory shear measurements. The critical gel point was determined according to Winter's criterion: 1) from the measurements of storage and loss moduli as a function of heating time at different frequencies, and 2) from the exponents of the power law mechanical spectra obtained after cure experiments performed up to a maximum temperature (60-90 degreesC) followed by a sudden decrease in temperature up to 20 degreesC. Differential Scanning Calorimetry (DSC) was performed in order to investigate thermal transitions in egg yolk. Microstructure of gels was evaluated by Transmission and Scanning Electron Microscopy. The results obtained were discussed in terms of the processes involved in protein gelation: change in the protein system, aggregation of partially denaturated protein molecules and association of aggregates. As a result, an elastic gel network was always obtained. The influence of frequency, heating rate, solids concentration and maximum temperature of processing, was analysed. Most of the transformations found during thermal processing were found to be basically irreversible, even at the sol state and gel point. However, some reversible phenomena were detected during constant temperature processing depending on the maximum temperature performed.

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