期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 113, 期 -, 页码 173-180出版社
HUMANA PRESS INC
DOI: 10.1385/ABAB:113:1-3:173
关键词
solid-state fermentation; lipase; Penicillium; soy cake; olive oil; moisture
The production of lipases by Penicillium simplicissimum using solid-state fermentation and soy cake as substrate was investigated. The effects of temperature, cake moisture, and carbon supplementation on lipase production were studied using a two-level experimental plan. Moisture, pH, and lipase activity were followed during fermentation. Statistical analysis of the results was performed to evaluate the effect of the studied variables on the maximum lipase activity. Incubation temperature was the variable that most affected enzyme activity, showing a negative effect. Moisture and carbon supplementation presented a positive effect on activity. It was possible to obtain lipase activity as high as 21 U/g of dry cake in the studied range of process variables.
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