4.7 Article

Identification and Characterization of Terpene Synthases Potentially Involved in the Formation of Volatile Terpenes in Carrot (Daucus carota L.) Roots

期刊

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 63, 期 19, 页码 4870-4878

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b00546

关键词

Daucus carota; terpenes; (E)-beta-caryophyllene; geraniol; terpene synthase

资金

  1. United States-Israel Binational Agricultural Research and Development Fund (BARD) [IS-4745-14R]
  2. ARS [ARS-0425034, 813495] Funding Source: Federal RePORTER

向作者/读者索取更多资源

Plants produce an excess of volatile organic compounds, which are important in determining the quality and nutraceutical properties of fruit and root crops, including the taste and aroma of carrots (Daucus carota L.). A combined chemical, biochemical, and molecular study was conducted to evaluate the differential accumulation of volatile terpenes in a diverse collection of fresh carrots (D. carota L.). Here, we report on a transcriptome-based identification and functional characterization of two carrot terpene synthases, the sesquiterpene synthase, DcTPS1, and the monoterpene synthase, DcTPS2. Recombinant DcTPS1 protein produces mainly (E)-beta-caryophyllene, the predominant sesquiterpene in carrot roots, and a-humulene, while recombinant DcTPS2 functions as a monoterpene synthase with geraniol as the main product Both genes are differentially transcribed in different cultivars and during carrot root development. Our results suggest a role for DcTPS genes in Carrot aroma biosynthesis.

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