期刊
ZOOLOGICAL SCIENCE
卷 21, 期 3, 页码 245-250出版社
ZOOLOGICAL SOC JAPAN
DOI: 10.2108/zsj.21.245
关键词
G-protein coupled receptor; octopus rhodopsin; metarhodopsin; G-protein; agonist
类别
资金
- NEI NIH HHS [EY01323] Funding Source: Medline
G-protein coupled receptors (GPCRs) mediate responses to many types of extracellular signals. So far, bovine rhodopsin, the inactive form of a GPCR, is the only member of the family whose three dimensional structure has been determined. It would be desirable to determine the structure of the active form of a GPCR. In this paper, we report the large scale preparation of a stable, homogenous species, truncated octopus rhodopsin (t-rhodopsin) in which proteolysis has removed the proline-rich C-terminal; this species retains the spectral properties and the ability for light-induced G-protein activation of unproteolyzed octopus rhodopsin. Moreover, starting from this species we can prepare a pure, active form of pigment, octopus t-Acid Metarhodopsin which has an all-trans-retinal as its agonist. Photoisomerization of t-Acid Metarhodopsin leads back to the inactive form, t-rhodopsin with the inverse agonist 11-cis-retinal. Octopus t-Acid Metarhodopsin can activate an endogenous octopus G-protein in the dark and this activity is reduced by irradiation with orange light which photo regenerates t-Acid Metarhodopsin back to the initial species, t-rhodopsin.
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