Effect of herbicide clomazone on photosynthetic processes in primary barley (Hordeum vulgare L.) leaves

The effect of pre-emergently applied herbicide clomazone on the photosynthetic apparatus of primary barley leaves (Hordeum vulgare L.) was studied. Clomazone application caused a reduction in chlorophyll (a + b) and carotenoid levels that was accompanied by a decline in the content of light harvesting complexes as judged from the increasing chlorophyll a/b ratio. The pigment reduction also resulted in changes in 77 K chlorophyll fluorescence emission spectra indicating lower chlorophyll (Chl) fluorescence reabsorption and absence of the long-wavelength emission forms of photosystem I. The maximal photochemical yield of photosystem II (PSII) and the reoxidation kinetics of the primary quinone acceptor Q(A)(-) were not significantly influenced by clomazone. A higher initial slope of Chl fluorescence rise in the Chl fluorescence induction kinetic indicated an increased delivery of excitations to PSII. Simultaneously, analysis of the Chl fluorescence quenching revealed that clomazone reduced function of the electron transport chain behind PSII. The decrease in the saturation rates of CO2 assimilation paralleled the decrease of the Chl content and has been suggested to be caused by a suppressed number of the electron transport chains in the thylakoid membranes or by their decreased functionality. The obtained results are discussed in view of physiological similarity of the clomazone effect with changes of photosynthetic apparatus during photoadaptation. (C) 2004 Elsevier Inc. All rights reserved.