期刊
PARASITE
卷 11, 期 1, 页码 103-106出版社
EDP SCIENCES S A
DOI: 10.1051/parasite/2004111103
关键词
Trypanosama vivax; cyclophosphamide; isolation; cryopreservation; Percoll; rodents
类别
A rodent adapted done of Trypanosoma vivax was used to infect cyclophosphamide treated mice and rats. Fresh blood containing trypanosomes, was centrifuged in a density gradient of three Percoll solutions, 1.07, 1.06, 1.05 g/ml, respectively, carefully layered on top of each other The yields of this simple procedure for trypanosome purification were about six times higher than those obtained with the conventional anion-exchange columns. Cryopreservation of trypanosomes using glycerol yieded 90% viable parasites, whereas using dimethylsulfoxide, a more commonly used cryoprotectant, the viability was only 35%.
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