期刊
MOLECULAR ECOLOGY NOTES
卷 4, 期 1, 页码 26-29出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1471-8286.2003.00554.x
关键词
cutthroat; hybridization; rainbow; species-specific markers
Eight polymerase chain reaction primer sets amplifying bi-parentally inherited species-specific markers were developed that differentiate between rainbow trout (Oncorhynchus mykiss) and various cutthroat trout (O. clarki) subspecies. The primers were tested within known F-1 and first generation hybrid backcrosses and were shown to amplify codominantly within hybrids. Heterozygous individuals also amplified a slower migrating band that was a heteroduplex, caused by the annealing of polymerase chain reaction products from both species. These primer sets have numerous advantages for native cutthroat trout conservation including statistical genetic analyses of known crosses and simple hybrid identification.
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