期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 91, 期 1, 页码 167-170出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2003.12.016
关键词
Zataria multiflora boiss; antinociceptive activity; fractionation; herbal medicine
Activity-guided fractionation of methanol: chloroform (1:1) extract of Zataria multiflora Boiss was performed in order to investigate the active component(s) responsible for antinociceptive activity. Antinociceptive activity was evaluated using hot-plate and writhing tests. Morphine (10 mg/kg, i.p.) and diclofenac (10 mg/kg, i.p.) were used as positive controls. The methanol-chloroform (1:1) extract of the aerial parts of the plants was prepared and partitioned between methanol-water (9:1) and n-hexane. The more active hydroalcoholic fraction was concentrated and further partitioned between methanol-water (3:2) and chloroform. The hydroalcoholic layer showed a dose-dependent and significant activity compared to morphine and diclofenac. Further fractionation of the hydroalcoholic fraction on silica gel gave six fractions. Three fractions at 100 mg/kg showed activity comparable to those of morphine. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
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