期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 540, 期 1-2, 页码 19-25出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2013.09.018
关键词
UDCA; NaDOC; Intestinal Ca2+ absorption; Ca2+-ATPase; Na+/Ca2+ exchanger; Calbindin D-28k
资金
- CONICET [PIP 2010-2012]
- Ministerio de Ciencia y Tecnica de la Provincia de Cordoba [PID 2010]
- SECyT (Universidad Nacional de Cordoba)
The aim of this study was to investigate the effect of ursodeoxycholic acid (UDCA) on intestinal Ca2+ absorption and to find out whether the inhibition of this process caused by NaDOC could be prevented by UDCA. Chicks were employed and divided into four groups: (a) controls, (b) treated with 10 mM NaDOC, (c) treated with 60 mu g UDCA/100 g of b.w., and (d) treated with 10 mM NaDOC and 60 mu g UDCA/100 g of b.w. UDCA enhanced intestinal Ca2+ absorption, which was time and dose-dependent. UDCA avoided the inhibition of intestinal Ca2+ absorption caused by NaDOC. Both bile acids altered protein and gene expression of molecules involved in the transcellular pathway of intestinal Ca2+ absorption, but in the opposite way. UDCA aborted the oxidative stress produced by NaDOC in the intestine. UDCA and UDCA plus NaDOC increased vitamin D receptor protein expression. In conclusion, UDCA is a beneficial bile acid for intestinal Ca2+ absorption. Contrarily, NaDOC inhibits the intestinal cation absorption through triggering oxidative stress. The use of UDCA in patients with cholestasis would be benefited because of the protective effect on the intestinal Ca2+ absorption, avoiding the inhibition caused by hydrophobic bile acids and neutralizing the oxidative stress. (C) 2013 Elsevier Inc. All rights reserved.
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