期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 529, 期 1, 页码 45-54出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2012.10.012
关键词
Mitochondrial oxidative stress; Submitochondrial particles; Manganese porphyrin; Free radicals; Superoxide radical; Antioxidants
资金
- Agencia Nacional de Investigacion e Innovacion (ANII), Uruguay
- CSIC-Universidad de la Republica, Uruguay
- National Institutes of Health
Peroxynitrite (ONOO-) formation in mitochondria may be favored due to the constant supply of superoxide radical (O-2(-)) by the electron transport chain plus the facile diffusion of nitric oxide (NO) to this organelle. Herein, a model system of submitochondrial particles (SMP) in the presence of succinate plus the respiratory inhibitor antimycin A (to increase O-2(-) rates) and the NO-donor NOC-7 was studied to directly establish and quantitate peroxynitrite by a multiplicity of methods including chemiluminescence, fluorescence and immunochemical analysis. While all the tested probes revealed peroxynitrite at near stoichiometric levels with respect to its precursor radicals, coumarin boronic acid (a probe that directly reacts with peroxynitrite) had the more straightforward oxidation profile from O-2(-)-forming SMP as a function of the NO flux. Interestingly, immunospintrapping studies verified protein radical generation in SMP by peroxynitrite. Substrate-supplemented SMP also reduced Mn(III)porphyrins (MnP) to Mn(II)P under physiologically-relevant oxygen levels (3-30 mu M); then, Mn(II)P were capable to reduce peroxynitrite and protect SMP from the inhibition of complex I-dependent oxygen consumption and protein radical formation and nitration of membranes. The data directly support the formation of peroxynitrite in mitochondria and demonstrate that MnP can undergo a catalytic redox cycle to neutralize peroxynitrite-dependent mitochondrial oxidative damage. (C) 2012 Elsevier Inc. All rights reserved.
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