期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 519, 期 1, 页码 32-37出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2012.01.006
关键词
Carotenoids; FastStep (TM); Macula; Retina; Surface plasmon resonance; Xanthophylls
资金
- National Institute of Health [EY-11600]
- Macula Vision Research Foundation
- Lowy Foundation
- Research to Prevent Blindness
- Foundation Fighting Blindness
The xanthophyll carotenoids lutein and zeaxanthin constitute the major carotenoids of the macular pigment in the human retina where they are thought to act in part to prevent light induced oxidative damage associated with age-related macular degeneration (AMD). The highly selective uptake of these pigments is mediated by specific carotenoid-binding proteins (GSTP1 and StARD3) recently identified in our laboratory. Carotenoids are hydrophobic in nature, so we first systematically optimized carotenoid preparations that are nano-dispersed in aqueous buffers, and then we used a new-generation surface plasmon resonance (SPR) protocol called FastStep (TM), which is significantly faster than conventional SPR assays. We have explored carotenoid-binding interactions of five proteins: human serum albumin (HSA), beta-lactoglobulin (LG), steroidogenic acute regulatory domain proteins (StARD1, StARD3) and glutathione S- transferase Pi isoform (GSTP1). HSA and LG showed relatively weak interaction with carotenoids (K-D > 1 mu M). GSTP1 evidenced high affinity and specificity towards zeaxanthin and meso-zeaxanthin with KD values 0.14 +/- 0.02 mu M and 0.17 +/- 0.02 mu M, respectively. StARD3 expressed a relative high specificity towards lutein with a K-D) value of 0.59 +/- 0.031 mu M, whereas StARD1 exhibited a relatively low selectivity and affinity (K-D > 1 mu M) towards the various carotenoids tested. Published by Elsevier Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据