期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 482, 期 1-2, 页码 1-6出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2008.12.003
关键词
Concanavalin A (ConA); Molecular modeling; Antiproliferative; Apoptosis; Caspase; Mitochondria
资金
- National Natural Science Foundation of China [30270331, 30670469]
- State Key Laboratory of Oral Diseases (Sichuan University).
The objective of this Study was to investigate the antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A (ConA) on human melanoma A375 cells. We firstly simulated the three-dimensional structure of ConA. Subsequently, we found that ConA possessed remarkable antiproliferative effect on A375 cells. Further experimental data indicated that there was a link between its hemagglutinating activity, mannose-binding activity and antiproliferative activity. In addition, we showed that ConA induced A375 cell apoptosis in a caspase-dependent manner. Then, we demonstrated that the treatment of ConA caused mitochondrial transmembrane potential (MMP) collapse, cytochrome c release and caspase activation. In conclusion, we report for the first time that there may be a close correlation between carbohydrate-binding activity of ConA and its antiproliferative activity. Also, we demonstrate firstly that ConA induces A375 cell death in a caspase-dependent manner as well as through a mitochondrial apoptotic pathway. (C) 2008 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据