Effect of nifedipine on capacitive calcium entry in Jurkat T lymphocytes

The effect of nifedipine-an antagonist of L-type calcium (Ca2+) channels-on capacitative Ca2+ entry (CCE) was studied in Jurkat T lymphocytes. CCE was induced by a variety of treatments each of which depleted intracellular Call stores. Cells were treated with thapsigargin, ionomycin, anti-CD3 antibodies, and phyto-haemagglutinin, or pre-incubated in a Ca2+-free medium. Activity of CCE was evaluated with a Ca2+-free/Ca2+-readmission protocol, in Fluo-3 pre-loaded cells. Nifedipine inhibited CCE in a dose-dependent manner. CCE inhibition was not due to non-specific effects on K+ channels. Nifedipine, did not induce any membrane depolarization, as revealed by measurements of the plasma membrane potential with the fluorescent probe bis-oxonol. Moreover, experiments done under depolarizing conditions (i.e. by substituting Na+ with K+ ions in the medium) revealed that nifedipine could inhibit capacitative Ca2+ entry independently of plasma membrane depolarization. We also demonstrated the presence in our Jurkat T-cells of transcripts for Ca(V)1.3 (alpha(1D)) and Ca(V)1.4 (alpha(1F)) L-type Ca2+ channels. Verapamil and diltiazem, two unrelated blockers of L-type Ca2+ channels, were less inhibitory on CCE. Possible mechanisms by which nifedipine interferes with Ca2+ entry in these cells are discussed. (C) 2008 Elsevier Inc. All rights reserved.