期刊
JOURNAL OF NEUROSCIENCE
卷 24, 期 9, 页码 2296-2303出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4439-03.2004
关键词
taste; sweet; preference; consumption; chorda tympani nerve; electrophysiology; genetics
资金
- NIAAA NIH HHS [R01 AA011028-08, R01 AA011028, AA11028] Funding Source: Medline
- NIDCD NIH HHS [DC03853, R01 DC000882-24, R01 DC000882, DC03509, DC04188, DC00882] Funding Source: Medline
- NIDDK NIH HHS [DK55853] Funding Source: Medline
Recent studies have shown that the T1R3 receptor protein encoded by the Tas1r3 gene is involved in transduction of sweet taste. To assess ligand specificity of the T1R3 receptor, we analyzed the association of Tas1r3 allelic variants with taste responses in mice. In the F-2 hybrids between the C57BL/6ByJ (B6) and 129P3/J ( 129) inbred mouse strains, we determined genotypes of markers on chromosome 4, where Tas1r3 resides, measured consumption of taste solutions presented in two-bottle preference tests, and recorded integrated responses of the chorda tympani gustatory nerve to lingual application of taste stimuli. For intakes and preferences, significant linkages to Tas1r3 were found for the sweeteners sucrose, saccharin, and D-phenylalanine but not glycine. For chorda tympani responses, significant linkages to Tas1r3 were found for the sweeteners sucrose, saccharin, D-phenylalanine, D-tryptophan, and SC-45647 but not glycine, L-proline, L-alanine, or L-glutamine. No linkages to distal chromosome 4 were detected for behavioral or neural responses to non-sweet quinine, citric acid, HCl, NaCl, KCl, monosodium glutamate, inosine 5'-monophosphate, or ammonium glutamate. These results demonstrate that allelic variation of the Tas1r3 gene affects gustatory neural and behavioral responses to some, but not all, sweeteners. This study describes the range of ligand sensitivity of the T1R3 receptor using an in vivo approach and, to our knowledge, is the first genetic mapping study of activity in gustatory nerves.
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