期刊
JOURNAL OF BIOTECHNOLOGY
卷 108, 期 2, 页码 153-159出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2003.11.010
关键词
17 beta-estradiol (E2); anti-E2 monoclonal antibody; bacterial magnetic particles (BMPs); fully automated immunoassay system
A fully automated immunoassay of 17beta-estradiol (E2) was performed using anti-E2 monoclonal antibody immobilized on bacterial magnetic particles (AntiE2-BMPs) and alkaline phosphatase-conjugated E2 (ALP-E2). E2 concentration in environmental water samples was evaluated by decrease in luminescence based on competitive reaction. A linear correlation between the luminescence intensity and E2 concentration was obtained between 0.5 and 5 ppb. The minimum detectable concentration of E2 was 20 ppt. All measurement steps were done within 0.5 h. The analysis of environmental water samples by a commercially available ELISA kit and the BMP-based immunoassay gave good correlation plots with a correlation efficient of 0.992. These results suggest that the fully automated system using the BMP-based immunoassay has some advantages in the high rapidity and sensitivity of the measurement. This system will enable us to determine low E2 concentrations without sample condensation. (C) 2003 Elsevier B.V. All rights reserved.
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