期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1028, 期 2, 页码 239-245出版社
ELSEVIER
DOI: 10.1016/j.chroma.2003.11.068
关键词
retention mechanisms; stationary phases; LC; mobile phase composition; anion-exchange; anions
We demonstrate in this report that a conventional silica-based strong anion-exchange column can exhibit reversed-phase chromatographic behavior simultaneously with ion-exchange in a methanol-aqueous phthalate mobile phase. Reversed-phase behavior is shown for PAHs in relatively high methanol-content mobile phases, while polar organics exhibit reversed-phase behavior in 0-10% (v/v) methanol-water eluents. At low concentrations (0-10% v/v) methanol has little or no effect on anion retention, while the anions exhibit only slight increases in retention in 60-80% (v/v) methanol at near-neutral pH values. Likewise, pH changes used to manipulate anion retention have essentially no effect on PAH retention, and cause only small decreases in retention for most of the polar organics studied. Little or no pH-effect was seen on a phenyl column (no exchange group) or with acetate mobile phase. It is shown that manipulation of pH and methanol content allows the grouping of neutral organic analytes early in a chromatogram, followed by the anions, with no class overlap. Several example chromatograms are given, including that of a red wine sample. Column efficiency was good for anions but only modest for organics. (C) 2004 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据