期刊
JOURNAL OF NEUROSCIENCE
卷 24, 期 10, 页码 2566-2574出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.5376-03.2004
关键词
neurotensin; dopamine; ventral tegmental area; patch clamp; intracellular calcium; calcium imaging; primary culture
Neurotensin (NT) increases neurotransmission within the mesolimbic dopamine system by enhancing the firing rate of dopaminergic (DAergic) neurons and by acting at the nerve terminal level. The signal transduction pathways involved in these effects have not been characterized, but NT receptors are coupled to the phospholipase C pathway and Ca2+ mobilization. However, an enhancement of intracellular Ca2+ concentration ([Ca2+](i)) evoked by NT in DAergic neurons has yet to be demonstrated. Furthermore, the hypothesis that the excitatory effects of NT in DAergic neurons are Ca2+ dependant is currently untested. In whole-cell recording experiments, DAergic neurons in culture were identified by their selective ability to express a cell-specific green fluorescent protein reporter construct. These experiments confirmed that NT increases firing rate in cultured DAergic neurons. This effect was Ca2+ dependent because it was blocked by intracellular dialysis with BAPTA. Using Ca2+ imaging, we showed that NT caused a rapid increase in [Ca2+](i) in DAergic neurons. Most of the Ca2+ originated from the extracellular medium. NT-induced excitation and Ca2+ influx were blocked by SR48692, an antagonist of the type 1 NT receptor. Blocking IP3 receptors using heparin prevented the excitatory effect of NT. Moreover, Zn2+ and SKF96365 both blocked the excitatory effect of NT, suggesting that nonselective cationic conductances are involved. Finally, although NT can also induce a rise in [Ca2+](i) in astrocytes, we find that NT-evoked excitation of DAergic neurons can occur independently of astrocyte activation.
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