Determination of nitrate in environmental water samples by conversion into nitrophenols and solid phase extraction-spectrophotometry, liquid chromatography or gas chromatography-mass spectrometry

Conversion of nitrate into a nitro-phenol derivative by reaction with 2-methylphenol or 2,6-dimethylphenol allowed at least 100-fold enrichment of the derivative on Lichrolut EN polymeric cartridge, and it is stable for up to 1 month on the cartridge. The derivative could be eluted with ammonia-methanol mixture. This reaction for nitrate determination has permitted a choice of final measurement by UV-Vis spectrophotometry, liquid chromatography or gas chromatography-mass spectrometry when the limits of detection were 10, 6 and 3 mug l(-1), respectively, and the calibration range 20 mug to 10 mg l(-1) nitrate. The method has been validated by spiking natural water samples, when the recovery of nitrate was 98.5-108.4% (relative standard deviation 2.5-6.1%). (C) 2003 Elsevier B.V. All rights reserved.