Immobilization of glucose oxidase onto electrochemically prepared poly(aniline-co-fluoroaniline) films

Poly(aniliwne-co-fluoroaniline) [poly(An-FAn)] films were electrochemically deposited on indium tin oxide glass plates. The characterization of these conducting copolymer films was carried out with ultraviolet-visible and Fourier transform infrared techniques. The enzyme glucose oxidase (GOX) was immobilized onto the conducting poly(An-FAn) films by a physical adsorption method. Amperometric response experiments carried out with poly(An-FAn)/GOX films revealed linearity from 0.5 to 22 mM glucose, and the Michaelis-Menten constant was found to be about 23.5 mM. The shelf life of these poly(An-FAn)/GOX electrodes was measured to be about 15 days, and the electrodes were stable up to 45degreesC. (C) 2004 Wiley Periodicals, Inc.