期刊
GENE
卷 328, 期 -, 页码 187-196出版社
ELSEVIER
DOI: 10.1016/j.gene.2003.12.013
关键词
SCC-112; cDNA and predicted amino acid sequences; subcellular localization; G2/M phase; cell death; tumor vs. normal tissue expression
资金
- NCI NIH HHS [CA68322, CA74175, P30-CA51008] Funding Source: Medline
We report here the identification and an initial characterization of a novel cell cycle-regulated molecule, SCC-112. SCC-112 cDNA (6744 bp) encodes a longest open reading frame (ORF) comprised of 1297 amino acids, representing a similar to 150-kDa nuclear protein. SCC-112 mRNA and protein levels were relatively high during the G2/M phase of the cell cycle in MDA-MB 435 breast cancer cells. Transient expression of SCC-112 cDNA in COS-1 cells led to an increase in the number of cells in sub-G1 phase and enhanced activity of caspase-3, a downstream effector of apoptosis. Stable transfection of SCC-112 cDNA in MDA-MB 231 breast cancer cells also led to an increase in the number of cells in sub-G1 phase (similar to 2-3-fold), indicative of apoptosis. The examination of the paired sets of human normal and tumor tissues revealed that the SCC-112 mRNA level was significantly high in normal breast and kidney tissues as compared to the corresponding primary tumor tissues (P<0.0001; breast, n=50, and kidney, n=20). Consistent with these observations, SCC-112 protein expression (150 kDa) was high in a majority of the normal renal tissues examined as compared to the matched renal tumor tissues (67%, 1.2-fold to > 10-fold, n= 18). Taken together, these findings suggest that the SCC-112 gene expression is likely to be associated with normal cell growth and proliferation. (C) 2004 Published by Elsevier B.V.
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