期刊
SCIENTIA HORTICULTURAE
卷 100, 期 1-4, 页码 215-227出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scienta.2003.08.009
关键词
choline dehydrogenase; Agrobacterium-mediated transformation; Brassica oleracea; salt stress; osmoprotectant
类别
The bacterial betA gene for biosynthesis, of glycinebetaine was transferred to cabbage (Brassica oleracea var. capitata) cultivar 'Golden Acre' through Agrobacterium-mediated transformation of hypocotyl explants. Transgenic status was established through Southern hybridization and mRNA expression in the shoots. The transformants exhibited higher tolerance to NaCl stress compared to untransformed parent plants. In physiological assessment of salinity tolerance, transgenics showed better growth response and greater stability in maintaining plant water relations at increasing levels of salinity. These results demonstrate that engineering glycinebetaine biosynthetic pathway into cabbage can lead to enhanced salt tolerance. (C) 2003 Published by Elsevier B.V.
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