期刊
ANALYTICA CHIMICA ACTA
卷 506, 期 2, 页码 133-135出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2003.11.009
关键词
hapten; polystyrene; silanization; ELISA
Anew method describing direct attachment of carboxylated haptens on a polystyrene support, using 3-aminopropyltriethoxysilane (3-APTES) as a linker, is reported. The hapten coated polystyrene support showed excellent stability as a function of the buffer pH and reaction time, and was successfully used to demonstrate its application in enzyme-linked immunosorbent assay (ELISA). (C) 2003 Elsevier B.V. All rights reserved.
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