期刊
JOURNAL OF CELL BIOLOGY
卷 164, 期 7, 页码 1033-1044出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200311023
关键词
calcium; PKC; oscillations; GFP; influx
类别
资金
- Wellcome Trust Funding Source: Medline
In mammalian eggs, sperm-induced Ca(2+) oscillations at fertilization are the primary trigger for egg activation and initiation of embryonic development. Identifying the downstream effectors that decode this unique Ca(2+) signal is essential to understand how the transition from egg to embryo is coordinated. Here, we investigated whether conventional PKCs (cPKCs) can decode Ca(2+) oscillations at fertilization. By monitoring the dynamics of GFP-labeled PKCalpha and PKCgamma in living mouse eggs, we demonstrate that cPKCs translocate to the egg membrane at fertilization following a pattern that is shaped by the amplitude, duration, and frequency of the Ca(2+) transients. In addition, we show that cPKC translocation is driven by the C2 domain when Ca(2+) concentration reaches 1-3 muM. Finally, we present evidence that one physiological function of activated cPKCs in fertilized eggs is to sustain long-lasting Ca(2+) oscillations, presumably via the regulation of store-operated Ca(2+) entry.
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