期刊
PROTEOMICS
卷 4, 期 4, 页码 1195-1203出版社
WILEY
DOI: 10.1002/pmic.200300677
关键词
apolipoprotein A-II; biomarkers; serum; surface-enhanced laser desorption; ionization; tumor
资金
- NCI NIH HHS [N01-CO-12400] Funding Source: Medline
A method to extract peptides and low molecular weight proteins from serum under denaturing conditions using acetonitrile containing 0.1 % trifluoroacetic acid has been developed. The extraction procedure precipitates large, abundant proteins to simplify subsequent mass spectral analysis. This sample preparation method provides an efficient way to extract serum peptides, enabling them to be compared and identified using different mass spectrometry approaches. Surface-enhanced laser desorption/ionization-time of flight mass spectrometry analysis of mouse blood serum samples prepared by this method allowed detection of two markers which were significantly reduced in mice with B cell lymphoma tumor. One of these markers has been identified as apolipoprotein A-II.
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