期刊
EMBO REPORTS
卷 5, 期 4, 页码 392-398出版社
WILEY-BLACKWELL
DOI: 10.1038/sj.embor.7400123
关键词
fitness; Cre/loxP; chemostat; adaptation; yeast
资金
- Wellcome Trust Funding Source: Medline
- Natural Environment Research Council [NE/B500190/1] Funding Source: researchfish
Yeast species have undergone extensive genome reorganization in their evolutionary history, including variations in chromosome number and large chromosomal rearrangements, such as translocations. To determine directly the contribution of chromosomal translocations to the whole organism's fitness, we devised a strategy to construct in Saccharomyces cerevisiae collinear, 'evolutionary mimics' of other species originally differing by the presence of reciprocal translocations in their genome. A modification of the Cre/loxP system was used to create in S. cerevisiae the translocations detected in the sibling species Saccharomyces mikatae IFO 1815 and 1816. Competition experiments under different physiological conditions showed that the translocated strains of S. cerevisiae consistently outcompeted the reference S. cerevisiae strain with no translocation, both in batch and chemostat culture, especially under glucose limitation. These results indicate that chromosomal translocations in Saccharomyces may have an adaptive significance, and lend support to a model of fixation by natural selection of reciprocal translocations in Saccharomyces species.
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