期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 161, 期 4, 页码 397-403出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1078/0176-1617-01237
关键词
osmotic stress; triphenyltetrazolium chloride; viability test; Zea mays callus; Zea mays roots
Live cells can reduce colorless 2,3,5-triphenyltetrazolium chloride (TTC) to a red insoluble compound, formazan. Maize (Zea mays) callus, when osmotically stressed by 0.53 mol/L mannitol, produced 7-times or more formazan than untreated control callus, This result was seen with all osmotica tested and could not be attributed to differences in TTC uptake rate or accumulation, increased respiration rate as measured by O-2 uptake, or to de,novo protein synthesis, Increased formazan production could be detected after 2.5 h of exposure to osmotic stress and leveled off after 48 h of exposure. The increased formazan production was only detected when callus was moved from high osmotic medium to low osmotic, TTC-containing medium. Abscisic acid increased TTC reduction only when added in combination with 0.53 mol/L mannitol. Incubation of maize seedling roots with 0.53mol/L mannitol also increased formazan produc ion as seen visually. Further studies are needed to determine the cause of the increased formazan production. These results show that TTC viability measurements must be carefully evaluated with appropriate controls to confirm their validity.
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