期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 42, 期 4, 页码 619-624出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2003.11.006
关键词
deoxynivalenol; nivalenol; acetyideoxynivalenols; trichothecenes; mycotoxins; De-epoxides; cytotoxicity; BrdU bioassay; 3T3 cells
The cytotoxicity of the de-epoxy metabolites of trichothecenes nivalenol (NIV) and deoxynivalenol (DON) was determined and compared with the cytotoxicity of the respective toxin with an intact epoxy group and their acetylated derivatives. The cytotoxic effects was determined by using the 5-bromo-2'-deoxyuridine (BrdU) incorporation assay assessing DNA-synthesis. The toxicity of NIV and DON expressed as the concentration inhibiting 50% of the DNA synthesis (IC50), was occurring at similar micromolar concentrations (1.19 +/- 0.06 and 1.50 +/- 0.34 muM). The toxicity of fusarenon X (4-acetyl NIV) in the assay was similar to the toxicity of NIV, and the toxicity of 15-AcDON was equal to the toxicity of DON. 3-AcDON was less toxic than DON and 15-AcDON. The IC50 value for de-epoxy DON was 54 times higher in the assay than the IC50 for DON, while the IC50 of de-epoxy NIV was 55 times higher than the IC50 for NIV. The results verify previous findings that the de-epoxidation is a detoxification reaction. (C) 2003 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据