期刊
STROKE
卷 35, 期 4, 页码 964-969出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/01.STR.0000119753.05670.F1
关键词
diabetes mellitus; insulin resistance; middle cerebral artery; potassium channels; oxidative stress; rats
资金
- NHLBI NIH HHS [HL-66074, HL-30260, HL-46558, HL-50587, HL-65380] Funding Source: Medline
Background and Purpose-Insulin resistance (IR) increases the risk of stroke in humans. One possible underlying factor is cerebrovascular dysfunction resulting from altered K+ channel function. Thus, the goal of this study was to examine K+ channel-mediated relaxation in IR cerebral arteries. Methods-Experiments were performed on pressurized isolated middle cerebral arteries (MCAs) from fructose-fed IR and control rats. Results-Dilator responses to iloprost, which are BKCa channel mediated, were reduced in the IR compared with control arteries (19+/-2% versus 33+/-2% at 10(-6) mol/L). Similarly, relaxation to the K-ATP opener pinacidil was diminished in the IR MCAs (17+/-2%) compared with controls (38+/-2% at 10(-5) mol/L). IR also reduced the K-ATP channel-dependent component in calcitonin gene-related peptide-induced dilation; however, the magnitude of the relaxation remained unchanged in IR because of a nonspecified K+ channel-mediated compensatory mechanism. In contrast, K-ir channel-mediated relaxation elicited by increases in extracellular [K+] (4 to 12 mmol/L) was similar in the control and IR arteries. Blockade of the K-ir and K-v channels with Ba2+ and 4-aminopyridine, respectively, constricted the MCAs in both experimental groups with no significant difference. Pretreatment of arteries with superoxide dismutase (200 U/mL) plus catalase (150 U/mL) restored the dilatory responses to iloprost and pinacidil in the IR arteries. Immunoblots showed that the expressions of the pore-forming subunits of the examined K+ channels are not altered by IR. Conclusions-IR induces a type-specific K+ channel dysfunction mediated by reactive oxygen species. The alteration of K-ATP and BKCa channel-dependent vascular responses may be responsible for the increased risk of cerebrovascular events in IR.
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