期刊
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
卷 36, 期 4, 页码 495-504出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.yjmcc.2004.01.005
关键词
heart; cellular electrophysiology; Cl- current; delayed rectifier K+ currents; pig atrial myocytes
Cellular electrophysiology is not fully understood in the atrium of pig heart. The objective of the present study was to determine whether transient outward current (I-to), ultra-rapid delayed rectifier potassium current (I-Kur), and rapid and slow delayed rectifier K+ currents (I-Kr and I-Ks) were present in pig atrium. The whole-cell patch technique was applied to record membrane currents and action potentials in myocytes isolated from pig atrium. It was found that an I-to was activated upon depolarization voltage steps to between -10 and +60 mV from -50 mV in pig atrial cells, and the I-to was sensitive to the inhibition by the blockade of L-type calcium (Ca2+) current, showed a bell-shaped I-V relationship, typical of I-to2 (i.e. I-Cl.Ca). The I-to2 was inhibited by the chloride (Cl-) channel blocker anthracene-9-carboxylic acid (9-AC, 200 mumol/l) or 4,4'-diisothiocyanostilben-2,2'disulfonic acid (200 mumol/l), and by Cl- substitution in the superfusate. I-Kur was found in pig atrial myocytes. and the current showed properties of weak inward rectification and use- and frequency-dependent reduction. I-Kur was resistant to tetraethylammonium. but sensitive to inhibition by 4-aminopyridine (4-AP) (IC50 = 71.7 +/- 3.5 mumol/l). In addition, E-4031-sensitive I-Kr and chromanol 293B-sensitive I-Ks were observed in pig atrial myocytes. Blockade of I-to2, I-Kur, I-Kr or I-Ks with corresponding blockers significantly prolonged atrial action potentials. These results indicate that Ca2+-activated I-to2, 4-AP-sensitive I-Kur, E-4031-sensitive I-Kr, and 293B-sensitive I-Ks are present in pig atrial myocytes, and these Currents play important roles in action potential repolarization of pig atria. (C) 2004 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据